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The screening of small molecule and ligand libraries that bind to protein targets in a high-throughput manner is an important, if not time-consuming,  part of drug discovery. The process often requires screening thousands of samples using a variety of assays over an extended period of time. Since protein targets are susceptible to degradation and aggregation, time is of the essence in protein stability screening.

Historically, protein interaction studies have been measured using an array of techniques, including nuclear magnetic resonance, differential scanning calorimetry, and surface plasma resonance imaging. Additionally, protein melt screening methods used in the past have been inefficient or expensive, capable of analyzing only small number of samples at time or requiring large quantities of protein samples, leading to increased costs per assay. Modern thermal shift assays bridge the gap between time-consuming sensitive assays and high-throughput screening platforms that are essential for the fast-paced nature
of drug library screening.

In this GEN webinar, Rama Badugu, Ph.D., Application Support Consultant for Roche Life Sciences, will describe the use of thermal shift assays, also called differential scanning fluorimetry, for protein stability and ligand screening. Furthermore, Dr. Badugu will highlight how these assays can be applied in high-throughput screening manner using the Roche LightCycler® 1536 real-time PCR system.

Who Should Attend

  • R&D scientists
  • Drug discovery researchers
  • Investigators studying protein-protein interactions
  • High-throughput screening scientists
  • Protein chemists
  • Small molecule researchers

You Will Learn

  • The basics of thermal shift assays and how they are applicable to drug discovery.
  • The advantage of thermal shift assays over traditional screening methods for proteinprotein or protein-ligand interactions.
  • How to utilize the Roche LightCycler 1536 as a high-throughput screening apparatus using the thermal shift assay technique.

Produced with support from:

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Rama Badugu, Ph.D.
Application Support Consultant
Roche Life Science, Roche Diagnostics