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We experience life in three dimensions, and within the past several years many aspects of research science are beginning to explore this approach. Two-dimensional cell culture has been indispensable in expanding our knowledge base in areas as diverse as developmental biology, cancer research, and drug discovery. However, the inability of cells in 2D to recapitulate the architecture and complex cellular matrices typically present in an in vivo environment often limits the amount of valuable information obtainable from cell culture assays.

The use of 3D cell cultures has been rising sharply in recent years from its initial introduction, over two decades ago. These 3D cultures more accurately represent the environment cells experience within a living organism. Moreover, these cultures can be utilized when studying various forms of cancer to foster the growth of organoids that can replicate key properties of in vivo organ systems or the original tumors from which they were derived. An additional benefit is that many 3D cultures are amenable to large-scale drug screens for the rapid detection of phenotypic or genetic changes associated with therapeutic compounds—an approach that opens the door for 3D culture use as an integral part of personalized medicine.

In this GEN webinar, our panelists will discuss how their methods and approaches to 3D cell culture have facilitated their current research projects.

Marshall Kosovsky, Ph.D., Global Scientific Support Manager for Corning Life Sciences, will give a brief introduction into advances in Corning’s 3D culturing solutions.

Ömer H. Yilmaz, M.D., Ph.D., Assistant Professor of Biology at the Massachusetts Institute of Technology, will describe his work on how adult stem cells and their microenvironment adapt to diverse conditions within the context of tissue regeneration and cancer initiation through the use of ex vivo intestinal organoid assays.

Wojciech Senkowski, Doctoral candidate in the Department of Medical Sciences at Uppsala University in Sweden, will discuss his current work, which looks for applications of various tumor spheroid models in high-throughput drug screening for ways to identify novel compounds that target these cell populations.

Who Should Attend

  • Cancer researchers
  • Scientists interested in or already utilizing 3D culture
  • Cell biologists
  • High-throughput screening scientists
  • Drug discovery researchers

You Will Learn

  • New methods for 3D cell culturing and how to apply them to high-throughput screening assays
  • About a novel multicellular tumor spheroid-based high-throughput drug screening solution
  • How organoid assays are aiding researchers in understanding the intricacies of ailments such as cancer and inflammatory bowel disease

Produced with support from:


Marshall Kosovsky, Ph.D.
Global Scientific Support Manager,
Corning Life Sciences

Ömer H. Yilmaz, M.D., Ph.D.
Assistant Professor of Biology,
Massachusetts Institute of Technology

Wojciech Senkowski
Doctoral Candidate,
Uppsala University, Sweden