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Traditionally, UV/Vis detection has been the workhorse of biopharmaceutical HPLC analysis, since many proteins and active biopharmaceutical ingredients typically possess a detectable chromophore. However, there are many instances where, although the biotherapeutic agent itself may be UV active, it has critical non-chromophoric chemical modifications and/or is formulated in UV-transparent excipients that must be detected and measured. In contrast, Charged Aerosol Detection (CAD) is a mass-based technique that provides a sensitive, near universal response for any nonvolatile analyte, independently of chemical composition. Consequently, it is able to detect substances unseen by UV absorption and provides valuable orthogonal data. CAD is well suited for the detection of substances without chromophores and the quantification of unknowns.

Join this webinar to hear several examples where charged aerosol detection can be used, from drug discovery to quality control, to detect and quantify substances separated by reversed phase and hydrophilic interaction chromatography. Examples include O- and N-linked glycans, sialic acids, surfactants, adjuvants, counter ions, excipients, sugars and amino acids. In many cases, detection limits are in the low nanogram range with peak area precision <3% RSD.

Who Should Attend

  • Biopharmaceutical analysts
  • HPLC laboratory managers
  • Analytical chemists
  • Biochemists
  • Biotechnologists

You Will Learn

  • An explanation of charged aerosol detection.
  • How CAD compares to other HPLC detection methods.
  • About selected applications in biopharmaceutical analysis.
  • How this technology can complement your analytic workflow.

Produced with support from:

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Dave Thomas, Ph.D.
Product and Support Scientist,
Thermo Fisher Scientific

Bruce Bailey, Ph.D.
Product Applications Manager,
Thermo Fisher Scientific