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Altered DNA methylation is an important regulator of gene expression contributing to a wide range of normal and disease phenotypes. While  the number of whole genome bisulfite sequencing studies is rapidly  growing, the sequencing costs associated with such studies can be  quite large. Reduced Representation Bisulfite Sequencing (RRBS) allows researchers to obtain quantitative DNA methylation information across many important features of the genome with approximately 50-fold  fewer sequencing reads than are needed with whole genome bisulfite  sequencing, resulting in substantial sequencing cost reduction.

During this webinar we will describe and present data from a novel  approach to RRBS. The Ovation® RRBS Methyl-Seq System can be used to  generate RRBS libraries suitable for sequencing on Illumina platforms  in a single day using 100 ng of human genomic DNA without gel-based size selection. The approach overcomes two challenges traditionally  associated with the RRBS approach, namely the problem of low base  diversity and the inability to identify PCR duplicates. Both problems  have now been surmounted, allowing researchers to easily generate  high-quality RRBS data.

Who Should Attend

  • Cancer researchers
  • R&D scientists
  • Clinical research and development scientists
  • Researchers interested in epigenetics

You Will Learn

  • How RRBS can be used as a cost-effective method to survey samples for differential DNA methylation.
  • How the Ovation RRBS Methyl-Seq System overcomes the problem of low base diversity and eliminates the need for PhiX controls.
  • How the duplicate-marking capability used in the Ovation RRBS Methyl-Seq System enables an important data quality control, especially when working with limited or degraded samples.

Produced with support from:

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Ben Schroeder, Ph.D.
Senior Scientist,
NuGEN Technologies

Steven Kain, Ph.D.
NuGEN Technologies