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April 10, 2014

New Fluorophores

A review of literature describing the development of fluorogenic probes for live-cell imaging of intracellular proteins.

New Fluorophores

The authors of the article were able to achieve specific intracellular protein labeling with rapid kinetics and fluorogenic response through the design of a new PYP-tag ligand. [Sebastian Kaulitzki - Fotolia.com]

Studying intracellular proteins through fluorescence is ideally performed in live cells in a rapid manner The use of fluorescent proteins such as green fluorescent protein (GFP) has been widely applied using GFP-fusions to a protein of interest as well as split GFP systems to study intracellular protein–protein interactions This article* ...

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