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November 01, 2013 (Vol. 33, No. 19)

PCR @ 30: 30 Years Young and Still Evolving

  • It was 30 years ago when Kary Mullis conceived the chain reaction that looked possible by adding polymerase to a DNA molecule in the presence of short oligonucleotide primers and nucleotide triphosphates. Cycling the temperature to sequentially denature the double-stranded DNA template, anneal primers to the template, and ...

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