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October 01, 2009 (Vol. 29, No. 17)

Optimizing CHO Cell Culture Conditions

Temperature, Impeller Speed, pH, and DO Parameters Set Up to Maximize Productivity

  • Comparison with Shake Flasks

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    Figure 3. Comparison of batch cultures of CHO cells in shake flasks (shake) and the Dasgip Parallel Bioreactor System without (bioreactor) and with (DO-controlled bioreactor) a 30% minimum DO set point

    Shake flasks are routinely used for growth of cell lines and are generally believed to provide a reasonable estimate of cell-line behavior in the first days of growth in a bioreactor. Comparison of CHO cells grown in the Dasgip Parallel Bioreactor System to cells grown in shake flasks demonstrated that the growth and antibody-production characteristics over the first five days of culture were similar for shake flasks and the non-DO controlled bioreactor (Figures 3A and 3B). However, the increased DO concentration in the medium in the DO-controlled bioreactor resulted in increased antibody production.

    Shake flasks fail to maintain the appropriate aeration to support batch culture viability beyond day seven, and therefore it is likely that the growth of cells in the Dasgip Parallel Bioreactor System will provide a better scale-down model for comparison to large-scale fermentation over the duration of a complete batch culture (batch or fed-batch). The design features of the Dasgip Parallel Bioreactor System combines precise control of the culture conditions with ease of use and gives data that offers predictive information which is directly relevant to designing large-scale culture processes. 

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