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January 01, 2011 (Vol. 31, No. 1)

Increasing Throughput in Cellular Assays

Reduction of Edge Effect Allows Results to Remain Consistent Across Entire Plate

  • Conclusion

    With the need to streamline processes by increasing throughput and automating protocols, it is vital that cell-culture plates are able to provide consistent and reliable results. Thus, the prevention of variation between the plates’ outer and inner wells allows results to remain more true to the population phenotype for more efficient cellular analysis.

    The Thermo Scientific Nunc Edge Plate greatly reduces the edge effect, which is commonly experienced during cell culture. By reducing well-to-well variability, results remain consistent across the entire plate, enabling the confident use of the outer and corner wells to increase throughput. The reduction in evaporation observed enables concentrations to remain consistent across all wells and efficient cell growth across the entire plate. Furthermore, the ability to fill the moat with agarose provides a degree of solidity, preventing spillages upon loading to automated platforms, while maintaining the same effect as obtained with water.

    Many cell-based assays require multiple targets to be simultaneously imaged, where all of the fluorescence from a single location needs to focus at the same point within the imaging system. Nunc Edge Plates are flat, minimizing the refocusing needed when sampling several fields per well, providing efficient automated imaging. As a result, users performing various high-content analyses can prepare cell cultures that remain healthy and viable across the Nunc Edge plate, while being imaged with ease.

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