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September 15, 2010 (Vol. 30, No. 16)

High-Velocity Affinity Chromatography

Silica-Based Protein A Media Designed to Reduce Bottlenecks in Antibody-Capture Process

  • Selectivity

    AbSolute silica-based media has the same selectivity as other types of media. For example, the purity of IgG eluted from AbSolute is similar to both the industrial reference and the IgG eluted from agarose-based protein A media.

  • Economics and Ease of Operation

    Modified silica-based protein A media is incompressible, meaning that a 1 L column is packed with 1 L of media. This feature is an improvement over soft gels such as agarose-based media that have shrink factors of 0.75 and require a higher quantity of media to pack a 1 L column.

    Moreover, silica-based media is easy to pack and can be used with most chromatography equipment (low, medium, and high pressure) from lab to production scale. For example, the packing of a 20 cm bed length in a 110 mm I.D. low pressure Prochrom® Bio column takes two minutes to obtain 5,900 plates at 160 cm/h.

    This comparative study illustrates the benefits of AbSolute modified silica-based protein A media in large-scale mAb production. Its use allows biomanufacturers to speed up their antibody-capture processes, making them more cost-effective.

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