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October 01, 2011 (Vol. 31, No. 17)

Evolution of Polymerase Chain Reaction

Seminal Technology Continues to Be a Work in Progress

  • Since the discovery of the polymerase chain reaction (PCR) by the oligonucleotide chemist Kary Mullis in 1983, the method has revolutionized molecular biology and clinical diagnostics. Before PCR, DNA amplification required multiple steps, including cloning into plasmids, insertion into bacteria, bacterial growth, isolation of plasmid DNA, and separation ...

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