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April 15, 2009 (Vol. 29, No. 8)

Breaking HTS Cell-Based Assay Bottlenecks

New Tool Designed to Provide High-Efficiency Transfections with any Molecule in all Cell Types

  • Other Agents

    The MaxCyte STX electroporation process makes it possible to load other agents into cells besides DNA, including labile mRNA and siRNA, proteins and protein complexes, cell lysates, and small molecules. The problem of RNA degradation is minimized by the rapid transfection process in which RNA is added to the buffer immediately prior to electroporation.

    Expression levels can be controlled by adjusting the loading agent concentration.  Transfecting multiple molecules in defined stoichiometric ratios to create specific complexes is also possible with the MaxCyte STX.

    The MaxCyte STX can remove time and cost bottlenecks for preparing cell-based assays for HTS campaigns by rapidly transfecting large batches of cells with high-efficiency, cell viability, consistency, and reproducibility. Furthermore, by enabling high efficiency transfection with any exogenous molecule into any cell, including human primary cells and stem cells difficult to transfect by standard methods, the MaxCyte STX may help HTS advance in a more biologically relevant direction.

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