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April 15, 2009 (Vol. 29, No. 8)

Breaking HTS Cell-Based Assay Bottlenecks

New Tool Designed to Provide High-Efficiency Transfections with any Molecule in all Cell Types

  • Transfecting Cell Lines

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    Figure 2. Using the MaxCyte STX, HEK 293 and Vero cells were transfected with eGFP plasmid DNA.

    The MaxCyte STX can transfect with high efficiency essentially any mammalian cell line, differentiated human primary cell type, or human stem cell of interest to HTS studies. The data in the Table is representative of the cell types that have been efficiently transfected using the MaxCyte STX.

    All electroporations use plasmid DNA encoding green fluorescent protein (GFP) as a marker of transfection efficiency (expressed as the percentage of GFP-positive cells at 24–48 hours post-transfection). Cell viability for the same period is the percentage of cells excluding propidium iodide (PI). For many cells, transfection efficiency and viability are often >90%. Figure 2 shows Vero and HEK293 cells that have been transfected with GFP plasmid; bright field and fluorescent images are shown 24 hours after electroporation with the MaxCyte STX.

    The MaxCyte STX can also be used to transfect primary cells and stem cells, which are difficult to transfect by other methods. These types of cells are increasingly being considered for use in cell-based assays because they are more biologically relevant than cell lines.

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