GEN Roundup: Multiplexed Immunoassays: Small Samples, Big Pictures
Taking in "Everything All at Once" Can Reveal Meaningful Biological Patterns and Drug Targets
10 Takeover Targets to Watch This Fall
Pace of Biopharma M&A Slows from First Half Stampede of Deals
This is Your Brain on DREADDs
Chemogenetics Provides a Noninvasive Tool for Precise Control of Neural Circuits
Brexit’s Effect on U.K. Life Sciences
Making the Nation a One-stop Regulatory Engine for Innovation
Pushing the Limits of Bottom-Up Proteomics
Using State-Of-The-Art Capillary UHPLC and Orbitrap Mass Spectrometry for Reproducible Quantitation of Proteomes
<Sponsored Content>Click Image To Enlarge +Thermo Fisher app note. Use link to the left to download this PDF.
Since its inception, bottom-up proteomics has aimed to identify and quantify the complete proteome from a cell, tissue, or whole organism. Although many advances have been made in the last 15 years, there are still three main challenges to overcome. The first is to obtain complete coverage of the proteome by identifying all the expressed proteins in a given time. The second is working with samples of limited amounts such as like clinical biopsies, and the third is achieving sufficient analytical throughput. Understanding the dynamics of the proteome requires analyzing it across different conditions and time points throughout the cellular life cycle. For many studies, this analysis needs to be performed in a high-throughput manner. To further complicate matters, being able to discriminate the most important proteins constituting a given cellular state requires accurate peptide measurement across several orders of magnitude.