May 15, 2018 (Vol. 38, No. 10)
URL:
http://www.crisprflydesign.org
Rating:
Strong Points: Nicely designed, good collection of open-access resources
Weak Points: None
Summary:
CRISPR has taken the field of biological research by storm. As CRISPR has (seemingly overnight) been adopted by many researchers around the world, it is important for scientists using the technology to share their experiences and resources with the greater scientific community. That is the general idea behind CRISPR Fly Design, an open-access resource for researchers using CRISPR in Drosophila. Based at the German Cancer Research Center in Heidelberg, the team behind CRISPR Fly Design posts information about fly stocks (spanning D. melanogaster and three other Drosophila species), guide RNA plasmids, and experimental protocols. The protocols section includes the topics: selecting an appropriate strategy, avoiding off-target effects, making guide RNA, and finding your mutants. The website also includes a blog (though it is somewhat infrequently updated) as well as a list of links to other CRISPR resources.
