November 1, 2013 (Vol. 33, No. 19)
Strong Points: Nice amount of background and practical information
Weak Points: Some resources limited to Life Technologies products
RT-PCR is a technique as useful as it is ambiguously named. In this case, I’m referring to real-time PCR, also known as quantitative PCR (qPCR), but not to be confused with reverse transcriptase PCR (also abbreviated as RT-PCR)…OK, got that? If you’re still confused, the real-time PCR app by Life Technologies will clear things up for you. This app is a great reference for all real-time PCR users, whether you are new to the technique or an old pro. A primary feature of the app is the real-time PCR handbook, which provides detailed background information about the technique, as well as practical information for experimental design, data analysis, and troubleshooting. The app also includes a reaction calculator tool as well as a video library and a set of reference documents (although these two latter resources are most useful if you use Life Technologies products).