Using Flow Cytometry to Speed Determination of Eukaryotic Genome Sizes and Cell Type-Specific Gene Expression

• Broadcast Date: Wednesday, February 1, 2012
• Time: 1:00 pm EST, 10:00 am PST

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AVAILABLE ON DEMAND

Flow cytometry provides a convenient and accessible method for analysis of plant genome sizes and for other comparative studies. However, application of this technique to plants has been limited, due in part to the need for sample preparation that yields particle suspensions without cell perturbation, the limited dynamic range of conventional flow cytometers, and the affordability and complexity of most flow cytometry platforms.

For example, C-values for only about 1% of angiosperms (flowering plants) have been reported, due mostly to the large C-value dynamic range observed among these plants, which spans a range of about 1,250 fold. For plants with undetermined C-values, this complicates analysis using flow cytometry since the range of possible C-values exceeds the dynamic range of many flow cytometers.

In this webinar, presenters will describe development of a method using the BD Accuri® C6 flow cytometer for eukaryotic genome determination and cell type specific gene expression quickly, affordably, and with a small system footprint. Use of this instrument will also be demonstrated for the combined analysis of nuclear C-values and transgenic expression of a nuclear-targeted version of the green fluorescent protein (GFP) placed under the regulation of cell type specific promoters.

In This Webinar You Will Learn

• How the easy to use BD Accuri® C6 cytometer speeds eukaryotic genomic analysis for researchers with virtually no flow experience.
• How flow cytometric DNA-content analysis of plant nuclei suspensions prepared by the homogenation method is performed on the BD Accuri C6, and the importance of this work in determining C-values for angiosperms.
• How unlike other flow cytometers the BD Accuri C6 is very easy to learn, use, and adopt—fitting most lab spaces and budget limitations.
• How the BD Accuri C6 enables a range of applications from plant cell genome size analysis to quantitation of reporter and reference plasmid expression at the single mammalian cell level.

Who Should Attend

• Molecular biologists
• Agricultural scientists
• Microbiologists
• Plant geneticists
• Crop development scientists 
• Molecular geneticists

Panelists Include

• Clare Rogers, Senior Marketing Applications Specialist, BD Biosciences-Cell Analysis
• Chuck Cannon, Ph.D., Associate Professor, Texas Tech University, Professor, Xishuangbanna Tropical Botanic Garden, Chinese Academy of Sciences
• David Galbraith, Ph.D., Professor, BIO5 Institute and School of Plant Sciences, University of Arizona

Tamlyn Oliver, Managing Editor, Genetic Engineering & Biotechnology News, will be the host of this webinar. A live Q&A session will follow the presentations, offering you a chance to pose questions to our expert panelists.

AVAILABLE ON DEMAND