Glycobiology Analysis Solutions Using HPAE-PAD

• Broadcast Date: Thursday, April 18, 2013
• Time: 8:00 am PDT, 11:00 am EDT, 16:00 BST

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Post-translational modification of proteins with oligosaccharides to form glycoproteins is a common biological motif. These glycoprotein oligosaccharides are involved in a wide range of biological and physiological processes including recognition and regulatory functions, cellular communication, gene expression, cellular immunity, growth, and development. Aberrant glycosylation of proteins is connected to cancer progression, invasion, and metastasis. With many biological functions, glycosylation is one of the most important post-translational modifications of eukaryotic cell proteins.

Oligosaccharides are covalently attached to proteins primarily through two structural motifs. They can be attached to the amide group of an asparagine, referred to as “N-linked glycans” or attached to proteins through the hydroxyl group on serine or threonine, referred to as “O-linked glycans”. The biological activity and function of N-linked glycans are well studied as compared to O-linked glycans. However, both types of glycans are investigated as biomarkers in order to understand changes related to complex organelle development, and as part of therapeutic protein drug development, which has increasing evidence that efficacy is effected by glycosylation.

There are many analytical approaches that have been employed in the area of carbohydrate analysis that can be generally categorized as monosaccharide analysis or oligosaccharide analysis after carbohydrate release from the protein of interest. This webinar will discuss how HPAE-PAD analyses are performed to determine monosaccharides, sialic acids, oligosaccharidesand other carbohydrate components of glycoproteins.

Key Learning Objectives:

• Learn about the importance of protein glycosylation and analysis challenges.
• Investigate methods for isolating and analyzing carbohydrate posttranslational modifications.
• Learn about HPAE-PAD and how it can be a powerful tool in carbohydrate analysis.
• See methods illustrating how HPAE-PAD can be effectively used to investigate key post-translational modifications.

Who Should Attend:

• R&D managers who want additional understanding of carbohydrate analysis.
• Product Development Scientists who need to become knowledgeable about methods for studying and identifying carbohydrates in proteins.
• Quality Control Professionals in the biotechnology industry.

A live Q&A session will follow the presentations, offering you a chance to pose questions to our expert panelists.

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