Cell-Based Assays for Enhanced Safety, Activity, and Target Specificity Determination
- Broadcast Date:
Tuesday, October 2, 2012
1 PM ET
REGISTRATION IS FREE
Given the importance of small molecule therapeutics in the treatment of several disease classes, increased focus has been placed on the development of robust assays to improve identification of safe, active, and specific compounds. For example, targeted kinase inhibitor discovery and development have increased greatly since these small molecules have provided effective therapeutics to treat several intractable cancers.
During the kinase drug discovery and development process, early identification of potential toxicities and off-target effects of these molecules has proven challenging, and physiologically relevant assays that can accurately correlate kinase in vitro and cellular functional activities have been lacking.
During this webinar, participants will discuss the development of cell-based assay platforms and their applications for early assessment of potential toxicity and drug promiscuity. First, the development and application of kinase inhibitor screening assays developed by PerkinElmer in collaboration with Pfizer will be discussed. These assays can detect and quantitate specific substrate phosphorylation, allow determination of biochemical to cellular translation of kinase activity, and improve compound profiling to determine selectivity.
Second, a review of PerkinElmer’s suite of cellular assay solutions will be followed by a discussion of homogeneous cellular assays for the detection of endogenous phosphorylation (developed by PerkinElmer in collaboration with TGR BioSciences). The value of utilizing cell-based kinase assays as a functional read-out of GPCR pathway activation will also be explored.
In This Webinar You Will Discover
- Why cell-based assays are a valuable tool in drug discovery for lead evaluation
- How cell-based kinase functional assays play an important role in drug safety and toxicology during kinase inhibitor drug development
- How kinase selectivity profiles can guide early safety assessments
- How cellular kinase assays can be used as functional assays for other target classes
- How a typical assay works
Who Should Attend
- Basic research scientists
- Drug development researchers
- Oncology researchers
- Pharmacology/toxicology researchers
- QC/QA scientists
- Assay development scientists
A live Q&A session will follow the presentations,
offering you a chance to pose questions to our expert panelists.