GEN UPDATES in biotechnology: Transfection

GEN Technology Updates are composed of articles commissioned for publication by Genetic Engineering & Biotechnology News and of peer-reviewed scientific papers from journals published by Mary Ann Liebert, Inc. They are intended to provide in-depth information and coverage on cutting-edge technologies and trends that are transforming drug discovery and life science research.

In Vitro Nucleic Acid Transfection Methods
Patricia F. Dimond, Ph.D.

Minimizing Off-Target Effects in Transfection
Jay Wang

Distal ERBB2 Promoter Fragment Displays Specific Transcriptional and Nuclear Binding Activities in ERBB2 Overexpressing Breast Cancer Cells
Laurence Delacroix, Dominique Begon, Guillaume
Chatel, Pascale Jackers, and Rosita Winkle

Development of Luciferase Reporter-Based Cell Assays
Chunfai Lai, Xin Jiang, and Xianqiang Li

Development of a Synthetic Promoter for Macrophage Gene Therapy
Weijing He, Mei Qiang, Wuqiong Ma, Anthony J. Valente, Marlon P. Quinones, Wen Wang, Robert L. Reddick, Qifu Xiao, Seema S. Ahuja, Robert A. Clark, Gregory L. Freeman, and Senlin Li

Interferon-Inducible Mx Gene Expression in Cotton Rats: Cloning, Characterization, and Expression During Influenza Viral Infection
Lioubov M. Pletneva, Otto Haller, David D. Porter, Gregory A. Prince, and Jorge C.G. Blanco

Abr and Bcr, Two Homologous Rac GTPase-Activating Proteins, Control Multiple Cellular Functions of Murine Macrophages
Young Jin Cho, Jess M. Cunnick, Sun-Ju Yi, Vesa Kaartinen, John Groffen, and Nora Heisterkamp Mol. Cell. Biol., Feb 2007; 27: 899 — 911

Granulogenesis in Non-neuroendocrine COS-7 Cell Induced by EGFP-tagged Chromogranin A Gene Transfection: Identical and Distinct Distribution of CgA and EGFP
Chie Inomoto, Shinobu Umemura, Noboru Egashira, Takeo Minematsu, Susumu Takekoshi, Yoshiko Itoh, Johbu Itoh, Laurent Taupenot, Daniel T. O'Connor, and R. Yoshiyuki Osamura J. Histochem. Cytochem., Jan 2007; 10.1369/jhc.6A7110.2007

Progesterone Receptors Upregulate Wnt-1 To Induce Epidermal Growth Factor Receptor Transactivation and c-Src-Dependent Sustained Activation of Erk1/2 Mitogen-Activated Protein Kinase in Breast Cancer Cells
Emily J. Faivre and Carol A. Lange Mol. Cell. Biol., Jan 2007; 27: 466— 480.

Prospective Early Response Imaging Biomarker for Neoadjuvant Breast Cancer Chemotherapy
Kuei C. Lee, Bradford A. Moffat, Anne F. Schott, Rachel Layman, Steven Ellingworth, Rebecca Juliar, Amjad P. Khan, Mark Helvie, Charles R. Meyer, Thomas L. Chenevert, Alnawaz Rehemtulla, and Brian D. Ross Clin. Cancer Res., Jan 2007; 13: 443—450.

Cases of Mistaken Identity
Chatterjee, R., 2007. Science, Vol. 315, pp 928—931

Comparison of established cell lines at different passages by karyotype and comparative genomic hybridization.
Wenger, S.L. et al. 2004. Bioscience Reports, 24 (6):631—639

Interpretation of cell culture phenomena.
Vierck J.L. et al. 2000. Methods in Cell Science, 22(1): 79—81

 

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