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  • Harnessing the Power of Kinase Inhibitors
    ... Chantry also notes that the alternative global approaches of molecular-driven target discovery, such as using an shRNA knockdown approach or systematic knockouts in animal models, ...
    5-8-2013
  • Advances in RNAi Tools and Technologies
    ... Integrated shRNA sequences are PCR amplified and amplicons are analyzed by next-generation ... By exploiting the cell's endogenous RNAi processing machinery, shRNA constructs allow for ...
    4-15-2013
  • NIDA Refocuses on Translational, Genetic Medicine
    identification of RNA and DNA sequences in formalin fixed nervous tissue, shRNA, microfluidics, epigenetics diagnostics, therapeutics, and tools to detect epigenetic modifications. ...
    12-6-2012
  • Overcoming Drawbacks of Gene Silencing with RNAi
    There are also risks, however, when using RNAi that need to be considered carefully. Figure 1. Screening of 10 shRNA sequences (shRNA1-10) for the silencing of Hsp90b: The shRNA5 ...
    12-1-2012
  • RNAi's Enabling Powers
    This approach is supported by protocols and a large portfolio of products, including shRNA ... Identifying the exact shRNA producing the phenotype is then determined using next-gen ...
    11-1-2012
  • Automation Redefines Compound Management
    ... Even though compound management is evolving quickly, new challenges are directly ahead. For example, nucleic acid reagents such as plasmids, siRNA, microRNA, and shRNA are ...
    10-1-2012
  • New Target for Acute Myeloid Leukemia Found
    Conversely, knockdown of HLX in both human and mouse AML cells using a targeted shRNA led to reduced cell proliferation, and increased survival following transplantation into ...
    8-14-2012
  • Breast Cancer Drug Resistance Linked to Gene Family
    ... And when breast cancer cells were treated with an shRNA that blocked FAM38A expression, the cells became less proliferative and more sensitive to EGFR-TKI treatment. Conversely, ...
    8-14-2012
  • Exposing the Roots of Chemotherapy Resistance
    that expressed a WNT16B-inhibiting shRNA. The results of this set of experiments ... those that expressed a WNT16B-silencing shRNA. Tumors growing in the presence of ...
    8-6-2012
  • Fighting Brain Tumors by Inhibiting MET
    ... This change in gene-expression patterns was blocked in HGF-stimulated VEGFKO cells transfected with an MET shRNA, again supporting a key role for MET activity. And although mice ...
    7-9-2012
  • 5 Tips to Improve Your RNAi Screens
    Cells transduced with an "empty" (no shRNA construct) or a nontargeting shRNA should be included in all experiments as controls. Such controls help account for any changes in gene ...
    6-20-2012
  • Leveraging the Cancer Transcriptome for New Drug Targets
    ... The investigators performed a large-scale shRNA loss-of-function screen to define genes upon which each of these distinct cell types specifically depend. They identified 15 genes ...
    6-20-2012
  • Capitalizing on miRNA Research
    ... Cellecta designs and constructs pooled libraries expressing short hairpin RNAs (shRNA) and ... growth and proliferation of cancer cells. shRNA consists of a sense strand, short loop ...
    6-15-2012
  • Studies Show JAK1/2 Inhibition Increases Tumor Cell Susceptibility...
    Medical School designed a lentiviral shRNA screen to identify which of more than ... The lentiviral library used for the shRNA screen was a kinase/phosphatase subset of The ...
    6-12-2012
  • Studies Find Melanoma Exosomes Educate Bone Marrow-Derived Cells...
    ... B16-F10 tumor cells in mice that had been pretreated with either unmodified B16-F10 exosomes or shRNA-knockdown B16-F10 exosomes that exhibited markedly reduced MET expression. ...
    5-24-2012
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    Journal Articles

  • Evaluation of Cyclin-Dependent Kinase-Like 1 Expression in Breast...
    Li Tang, Yanfang Gao, Feng Yan, Jinhai Tang
    Cancer Biotherapy and Radiopharmaceuticals
    The roles of CDKL1 in cell growth were analyzed with CDKL1 short hairpin RNA (shRNA) ... Inhibiting CDKL1 function with shRNA, MCF-7 cells exhibited obvious accumulation at the ...
  • Short Communication: A Single Step Assay for Rapid Evaluation...
    Ajit G. Chande, Masanori Baba, Robin Mukhopadhyaya
    AIDS Research and Human Retroviruses
    Iinhibition of luciferase reporter expression in a cell line harboring the plasmid in presence of tat targeted shRNA confirmed specificity of the assay and a dose-depended reporter ...
  • Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish
    Gianluca De Rienzo, Jennifer H. Gutzman, Hazel Sive
    Zebrafish
    Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish Zebrafish Despite the ... This included quantifying RNA knockdown, testing specificity of shRNA effects, and ...
  • Overcoming Paclitaxel Resistance in Lung Cancer Cells Via Dual...
    Zheng-Xiang Han, Hong-Mei Wang, Guan Jiang, Xiu-Ping Du, Xiang-Yang Gao, Dong-Sheng Pei
    Cancer Biotherapy and Radiopharmaceuticals
    In this study, we used a shRNA approach to evaluate the effect of STMN1 and Bcl-2 ... and Bcl-2 mRNA and protein expression by a combination of double shRNA treatment strategy. ...

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