Current Issue

View Larger Image

Past Issues Free Subscriptions
Sponsored
Keywords



Search Results - ShRNA
 More »

  • DNA Vaccines Free from Mobile DNA
    For example, plasmids with secondary structure such as viral Long Terminal Repeats (LTR) or short-hairpin RNA (shRNA) grow faster when the secondary structure (and its function) is ...
    10-1-2013
  • Literature Review: New Combination of Targets Shows Promise for...
    ... The synergy observed could also be replicated with kinase-dead CHK1 or CHK1 shRNA and the ... lines, while p53 knock-down by shRNA sensitized p53-wt cells to lethality of this regimen. ...
    8-20-2013
  • Harnessing the Power of Kinase Inhibitors
    ... Chantry also notes that the alternative global approaches of molecular-driven target discovery, such as using an shRNA knockdown approach or systematic knockouts in animal models, ...
    5-8-2013
  • Advances in RNAi Tools and Technologies
    ... Integrated shRNA sequences are PCR amplified and amplicons are analyzed by next-generation ... By exploiting the cell's endogenous RNAi processing machinery, shRNA constructs allow for ...
    4-15-2013
  • NIDA Refocuses on Translational, Genetic Medicine
    identification of RNA and DNA sequences in formalin fixed nervous tissue, shRNA, microfluidics, epigenetics diagnostics, therapeutics, and tools to detect epigenetic modifications. ...
    12-6-2012
  • Overcoming Drawbacks of Gene Silencing with RNAi
    There are also risks, however, when using RNAi that need to be considered carefully. Figure 1. Screening of 10 shRNA sequences (shRNA1-10) for the silencing of Hsp90b: The shRNA5 ...
    12-1-2012
  • RNAi's Enabling Powers
    This approach is supported by protocols and a large portfolio of products, including shRNA ... Identifying the exact shRNA producing the phenotype is then determined using next-gen ...
    11-1-2012
  • Automation Redefines Compound Management
    ... Even though compound management is evolving quickly, new challenges are directly ahead. For example, nucleic acid reagents such as plasmids, siRNA, microRNA, and shRNA are ...
    10-1-2012
  • New Target for Acute Myeloid Leukemia Found
    Conversely, knockdown of HLX in both human and mouse AML cells using a targeted shRNA led to reduced cell proliferation, and increased survival following transplantation into ...
    8-14-2012
  • Breast Cancer Drug Resistance Linked to Gene Family
    ... And when breast cancer cells were treated with an shRNA that blocked FAM38A expression, the cells became less proliferative and more sensitive to EGFR-TKI treatment. Conversely, ...
    8-14-2012
  • Exposing the Roots of Chemotherapy Resistance
    that expressed a WNT16B-inhibiting shRNA. The results of this set of experiments ... those that expressed a WNT16B-silencing shRNA. Tumors growing in the presence of ...
    8-6-2012
  • Fighting Brain Tumors by Inhibiting MET
    ... This change in gene-expression patterns was blocked in HGF-stimulated VEGFKO cells transfected with an MET shRNA, again supporting a key role for MET activity. And although mice ...
    7-9-2012
  • 5 Tips to Improve Your RNAi Screens
    Cells transduced with an "empty" (no shRNA construct) or a nontargeting shRNA should be included in all experiments as controls. Such controls help account for any changes in gene ...
    6-20-2012
  • Leveraging the Cancer Transcriptome for New Drug Targets
    ... The investigators performed a large-scale shRNA loss-of-function screen to define genes upon which each of these distinct cell types specifically depend. They identified 15 genes ...
    6-20-2012
  • Capitalizing on miRNA Research
    ... Cellecta designs and constructs pooled libraries expressing short hairpin RNAs (shRNA) and ... growth and proliferation of cancer cells. shRNA consists of a sense strand, short loop ...
    6-15-2012
  • More »

    Journal Articles

  • shRNA-Mediated Decreases in c-Met Levels Affect the Differentiation...
    Ivana Rosová, Daniel Link, Jan A. Nolta
    Tissue Engineering, Part A
    shRNA-Mediated Decreases in c-Met Levels Affect the Differentiation Potential of Human ... We now investigated the specific role of HGF/c-Met signaling in MSC function. An shRNA-med...
  • Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish
    Gianluca De Rienzo, Jennifer H. Gutzman, Hazel Sive
    Zebrafish
    Efficient shRNA-Mediated Inhibition of Gene Expression in Zebrafish Zebrafish Despite the ... This included quantifying RNA knockdown, testing specificity of shRNA effects, and ...
  • Lentiviral shRNA knockdown of macrophage inflammatory protein...
    Po-Chuan Shen, Chia-Sing Lu, Ai-Li Shiau, Che-Hsin Lee, I-Ming Jou, Jeng-Long Hsieh
    Human Gene Therapy and Part B: Methods
    Lentiviral shRNA knockdown of macrophage inflammatory protein-1γ ameliorates experimentally induced osteoarthritis in mice Human Gene Therapy and Part B: Methods
  • Adenovirus-Mediated Silencing of Huntingtin Expression by shRNA
    Bin Huang, Stefan Kochanek
    Human Gene Therapy and Part B: Methods
    Adenovirus-Mediated Silencing of Huntingtin Expression by shRNA Human Gene Therapy and ... Since hairpin small interference RNA (shRNA) technology allows inhibition of specific gene ...

GEN Webinars

View »

GEN Web Briefs

View »

GEN Poll

More » Poll Results » Archive »

Balancing Family and Career for Women Ph.D.s

Which changes made to academic culture are most likely to encourage women researchers to stay?

Suggest a Poll