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  • Mouse Arrays for qPCR
    The mouse genome has been added to the PrimePCR assays for qPCR. PrimePCR assays are real-time PCR primers that have been experimentally validated to ensure performance. Through a ...
    8-1-2013
  • Single-Cell Genomics Poised for Growth
    ... third step the DNA/RNA is analyzed using genomic technologies such as qPCR or sequencing. ... integrate downstream with its BioMark(tm) HD qPCR platform for gene expression analysis. ...
    8-1-2013
  • Just How Versatile Is the Polymerase Chain Reaction?
    Until recently, one quantitative form of PCR, known as qPCR, was heralded as the gold standard of nucleic acid detection. A technology that was first described in 1999 by ...
    7-25-2013
  • RT-PCR: Ready for Prime Time?
    real-time PCR (qPCR) has made it a choice tool for many molecular analysis applications. Because of its speed, sensitivity, and specificity, qPCR has been displacing ...
    7-23-2013
  • Inline Automation and Miniaturized Reaction Volumes for Real...
    Real-time polymerase chain reaction (qPCR) processing can be a labor-intensive process ... processing, gene expression, RT-PCR, and qPCR. What You Will Learn How the Nexar ...
    7-17-2013
  • Life Tech Gets Deeper into Dx Business with Merck Collaboration
    new diagnostics across a wide range of therapeutic areas and Life Tech instrument platforms that include next-generation sequencing, Sanger sequencing, qPCR, and flow cytometry. ...
    7-16-2013
  • Harnessing the Clinical Power of Single Cells
    ... and profiled via single-cell quantitative real-time polymerase chain reaction (qPCR). ... For instance, using "housekeeping" genes to normalize qPCR expression data does not make ...
    6-21-2013
  • 6 Considerations for Multiplex qPCR Setup
    Fotolia.com] In multiplex qPCR, multiple targets are amplified in a single reaction tube. ... The experimental design for multiplexing is more complicated than for single-reaction qPCR...
    5-13-2013
  • Companion Diagnostics: 52 Pick-Up
    ... Life will apply its Taqman® Array Card technology to develop a qPCR-based diagnostic assay for GSK's MAGE-A3 cancer immunotherapy candidate, designed to identify patients likely ...
    5-9-2013
  • Cancer Diagnostics, Pushing the Boundaries
    ... The authors show that compared to qPCR, deep amplicon sequencing of the FGFR3 gene yielded ... with sequencing vs. about 50% with qPCR. "The additional mutations detected in ...
    5-8-2013
  • Biomarkers Take Center Stage
    ... The technology increases the observable signal noise ratio from a few hundred to over 50,000, resulting in the observation of many lower-abundance serum proteins. Epigenetic qPCR ...
    5-1-2013
  • Gene Expression Analysis in Living Cells
    ... Correlates with qPCR Results Figure 3. Probe detection of mRNA levels correlates to RT-PCR data. mRNA levels of EGFR RNA (A) in HeLa and MCF-7 cells and FGF2 mRNA (B) in HUVEC and ...
    5-1-2013
  • An Array of Genetic Tools from Gene Link
    tools to calculate concentration for oligo resuspension and dilution, typical reaction and cycler conditions for PCR/qPCR, and a molarity calculator, among a few other tools. ...
    5-1-2013
  • Illumina Partnership Eyes Expanded Presence in China
    NuPCR(tm) reagents for qPCR provide what Illumina says is a specific, sensitive, and budget-friendly solution for analyzing gene expression using real-time PCR. Key to the NuPCR ...
    4-18-2013
  • Thermo Fisher Swallows Life Tech
    ... consumables and bioproduction businesses, partially offset by sales declines the company said it expected to see in its older line of SOLiD® sequencers and qPCR royalty revenue. ...
    4-15-2013
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    Journal Articles

  • A New Method for Quantitative Real-Time Polymerase Chain Reaction...
    Xiayu Rao, Dejian Lai, Xuelin Huang
    Journal of Computational Biology
    A New Method for Quantitative Real-Time Polymerase Chain Reaction Data Analysis Journal of Computational Biology Quantitative real-time polymerase chain reaction (qPCR) is a ...
  • Overexpression of TDRP1 Gene in Swine Testis Cell and Its Global...
    Manqing Liu, Wei Zhang, Wei Luo, Qinghua Nie
    DNA and Cell Biology
    of TDRP1 gene continued to increase at 12, 24, and 36 h post transfection, and then decreased at 48 h, as indicated by both fluorescence signal and quantitative PCR (qPCR). ...
  • Extracellular matrix domain formation as an indicator of chondrocyte...
    Ling Wu, Stephanie Gonzalez, Saumya Shah, Levon Kyupelyan, Frank A Petrigliano, David R. McAllister, John S Adams, Marcel Karperien, Tai-Lian Tuan, Paul Benya, Denis Evseenko
    Tissue Engineering, Part C: Methods
    Imaging flow cytometry, immunohistochemistry, qPCR and glycosaminoglycan (GAG) assays were performed to evaluate the differences between COL II domain forming and COL II domain ...
  • Ribosomal Protein L13a as a Reference Gene for Human Bone Marrow...
    Deborah Studer, Stefanie Lischer, Wolfram Jochum, Martin Ehrbar, Marcy Zenobi-Wong, Katharina Maniura-Weber
    Tissue Engineering, Part C: Methods
    transcription polymerase chain reaction (qPCR) is the method of choice to study changes ... RPL13a was validated for qPCR studies of MSCs (bone marrow- and placenta-derived) and ...

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