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  • 6 Considerations for Multiplex qPCR Setup
    Fotolia.com] In multiplex qPCR, multiple targets are amplified in a single reaction tube. ... The experimental design for multiplexing is more complicated than for single-reaction qPCR...
    5-13-2013
  • Companion Diagnostics: 52 Pick-Up
    ... Life will apply its Taqman® Array Card technology to develop a qPCR-based diagnostic assay for GSK's MAGE-A3 cancer immunotherapy candidate, designed to identify patients likely ...
    5-9-2013
  • Cancer Diagnostics, Pushing the Boundaries
    ... The authors show that compared to qPCR, deep amplicon sequencing of the FGFR3 gene yielded ... with sequencing vs. about 50% with qPCR. "The additional mutations detected in ...
    5-8-2013
  • Biomarkers Take Center Stage
    ... The technology increases the observable signal noise ratio from a few hundred to over 50,000, resulting in the observation of many lower-abundance serum proteins. Epigenetic qPCR ...
    5-1-2013
  • Gene Expression Analysis in Living Cells
    ... Correlates with qPCR Results Figure 3. Probe detection of mRNA levels correlates to RT-PCR data. mRNA levels of EGFR RNA (A) in HeLa and MCF-7 cells and FGF2 mRNA (B) in HUVEC and ...
    5-1-2013
  • An Array of Genetic Tools from Gene Link
    tools to calculate concentration for oligo resuspension and dilution, typical reaction and cycler conditions for PCR/qPCR, and a molarity calculator, among a few other tools. ...
    5-1-2013
  • Illumina Partnership Eyes Expanded Presence in China
    NuPCR(tm) reagents for qPCR provide what Illumina says is a specific, sensitive, and budget-friendly solution for analyzing gene expression using real-time PCR. Key to the NuPCR ...
    4-18-2013
  • Thermo Fisher Swallows Life Tech
    ... consumables and bioproduction businesses, partially offset by sales declines the company said it expected to see in its older line of SOLiD® sequencers and qPCR royalty revenue. ...
    4-15-2013
  • Ingenuity, Affymetrix Pair Products to Curb Microarray Bottlenecks
    quantitative real-time polymerase PCR (qPCR), annouced that it would complement its ... contract research for a range of real-time qPCR services including primer and probe ...
    4-8-2013
  • Media's Role in Cell Culture Success
    ... Manufacturers also are employing rapid qPCR methods to detect contaminants like mycoplasma and viruses. Want more on cell culture? Be sure to check out the Expert Tips " Best ...
    4-1-2013
  • Querying the Cancer Transcriptome
    ... Microarray analyses combined with RT-qPCR performed by Dr. Lu and colleagues revealed that the induction of p53 target genes occurs at a much larger scale than previously thought. ...
    4-1-2013
  • Flexible qPCR Assay Assembly
    quantitative polymerase chain reaction (qPCR) is widely used throughout drug discovery ... High operating costs and advances in qPCR instrumentation to enable higher density 384- ...
    4-1-2013
  • Advertorial: Swift Biosciences
    developed myT® Primers, a line of novel qPCR reagents that provide both high ... myT Primer technology in Vela's real-time qPCR-based IVD products for the detection of ...
    4-1-2013
  • Life Tech's Pleasanton, CA Facility Gets $18M MDx Makeover
    an additional 30,000 square feet will follow over the next one to three years, with the goal of producing regulatory-compliant materials for qPCR workflows for clinical use. ...
    3-28-2013
  • Economic Benefits of Cold Plasma Technology
    ... PCR. After generating a standard qPCR dose-response curve, scientists used the ... system as part of a high-throughput qPCR protocol in 384-well format, where ...
    3-15-2013
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    Journal Articles

  • Three-Dimensional In Vitro Tri-Culture Platform to Investigate...
    Taymour M. Hammoudi, Catherine A. Rivet, Melissa L. Kemp, Hang Lu, Johnna S. Temenoff
    Tissue Engineering, Part A
    Each cell type was analyzed separately with quantitative polymerase chain reaction (qPCR) and histochemistry for several mesenchymal lineage markers. Distinct expression dynamics ...
  • A Three-Dimensional Tubular Scaffold that Modulates the Osteogenic...
    Mani T. Valarmathi, Michael J. Yost, Richard L. Goodwin, Jay D. Potts
    Tissue Engineering, Part A
    These cells were subsequently processed for real-time reverse-transcriptase polymerase chain reaction (RT-qPCR), immunohistochemical, cytochemical, and biochemical analyses. ...
  • Matrix Metalloprotease Inhibitors Suppress Initiation and Progression...
    Helge Bertram, Stephane Boeuf, Jasper Wachters, Sandra Boehmer, Christian Heisel, Michael W. Hofmann, Dorothea Piecha, Wiltrud Richter
    Stem Cells and Development
    Protease inhibitors were added during MSC chondrogenesis and stage-specific markers were assessed by histology, qPCR, and ALP quantification. Chondrogenesis was little affected by ...
  • Weight Loss in Obese Men Is Associated with Increased Telomere...
    Nathan J. O'Callaghan, Peter M. Clifton, Manny Noakes, Michael Fenech
    Rejuvenation Research
    Weight was reduced by an average of 10.6 kg across the study. Telomere length, measured by quantitative real-time PCR (qPCR), was negatively correlated with body mass index (BMI) ...

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