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'pcr'
  • Genome Engineering: CRISPR Proving More User-Friendly
    September 1, 2017
    ... genomic DNA, PCR amplify the fragment of interest, and then sequence these fragments. ... (for the region of interest) with PCR. After PCR amplification, the DNA undergoes ...
  • Insights
    September 1, 2017
    ... and automated workflows for quantitative PCR, gene expression, copy number variation ... that combines digital PCR with simultaneous real-time data for counting and validation. ...
  • PCR-Based Liquid Biopsy Is a Simple and Sensitive Cancer Diagnostic...
    August 14, 2017
    GEN News Highlights More » August 14, 2017 PCR-Based Liquid Biopsy Is a Simple and ... DNA released from cancer cells into the blood using a relatively simple PCR based assay. ...
  • Addressing Cell-Line Contamination to Improve Data Reproducibility...
    August 1, 2017
    ... chain reaction (PCR) analysis are required to assure the absence of contamination. ... Unlike the culture method, however, DNA staining will detect all types of mycoplasma. PCR: ...
  • Biomarker Platforms Advance Immuno-Oncology
    August 1, 2017
    ... of genomic approaches, such as DNA-seq, RNA-seq, digital PCR, and microarray, as well as for the validation of antibodies used for detection of biomarkers with IHC," adds Dr. ...
  • Culling Information from the Tumor Microenvironment
    August 1, 2017
    ... Next, bead libraries are added to the cartridge. Each bead library contains a universal PCR priming site, a cell label, a UMI, and an mRNA capture sequence of oligo dT (Figure 1). ...
  • DNA Fab Keeps Getting More Fab
    August 1, 2017
    ... During the early 2000s, the main use of oligonucleotides was for PCR primers, and at that ... for a single project, all of which were unmodified PCR primers," recalls Dr. Behlke. ...
  • Insights
    July 1, 2017
    ... Under the agreement, the financial terms of which were not disclosed, the two companies will codevelop molecular diagnostic tests on the automated PCR-based Idylla platform, with ...
  • RNA-Seq: Less Lumping, More Splitting
    July 1, 2017
    ... Bunker notes, "but it resembles real-time PCR in terms of its simplicity and sensitivity." One of the applications of the RNAscope assay is to validate findings from single-cell ...
  • High-Definition Immunology
    June 15, 2017
    The system combines microfluidics with 5' molecular barcoding to enable V(D)J enrichment. The 5' barcoding avoids bias introduced by complex multiplex PCR, enables the detection of ...