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  • Harnessing the Power of Kinase Inhibitors
    His research team began by performing a dual transfection assay of catalytically inactive ... downstream effect of inhibiting NIK. As an alternative to the dual transfection assay, Dr. ...
    5-8-2013
  • Advances in RNAi Tools and Technologies
    ... Life Technologies provides a panel of lipid-based delivery reagents, such as Lipofectamine® RNAiMAX and Invivofectamine® 2.0, for transfection in cell culture or animal models, ...
    4-15-2013
  • Single-Use Bioreactor Roundup
    ... Manufacturing trends that continue to require SUB features include the renewed interest in transient transfection in process development, and continuous processing at the ...
    4-15-2013
  • Transfection Reagent
    TransIT®-BrCa transfection reagent is a dedicated breast cancer cell-line specific reagent that can help researchers achieve high expression levels in breast cancer cell types ...
    4-15-2013
  • Betting on Baculovirus Expression
    ... BacMam Technology Large-scale transient gene expression systems provide the ability for rapid production of recombinant proteins by transfection of the polyethyleneimine (PEI) ...
    3-15-2013
  • Overcoming Roadblocks in Cell-Based Assay Research
    ... Analyzer 2000. The same reagents for transfection and staining were used on a standard ... The same reagents for transfection and staining were used on a standard 384-well ...
    2-1-2013
  • Cell Electroporation System
    The Cellaxess® ACE adherent cell electroporation system allows for in situ transfection of any adherent cell type. The platform enables transfection and delivery of nongenetic ...
    2-1-2013
  • TAP Biosystems Partners with Life Tech on New Expression Platform
    The partnership will combine Life Technologies' Expi293(tm) expression system for transient transfection with TAP's ambr(tm) advanced micro bioreactor to enable automated ...
    1-15-2013
  • Sanford-Burnham Taps Intrexon to Speed Up iPSC Research
    than the standard method, and much faster than the standard two weeks of transfection. ... following as little as six days of transfection, although the yields were higher when ...
    1-3-2013
  • Tackling Transfection Tasks
    by target cells, referred to as transfection, which can be confounded by ... Similar to Inovio, Epivax' transfection method is to rely on electroporation to enhance ...
    1-1-2013
  • Better Control in Intracellular Flow Assays
    ... Techniques that create temporary holes in the membrane, used in the transfection of genetic material and in which cells can be maintained alive, are not suitable for intracellular ...
    1-1-2013
  • Tripping The Light Fantastic: New Reporter System
    ... where lane 1 is non-transfection control (transfection reagent only); lane 2, SV40-driven FLuc mono-reporter (pGL3-Control); and lane 3, FLuc-P2A-RLuc dual reporter (pCI-6.20). ...
    12-19-2012
  • Featured Video: The Art of Transfection
    This video from Life Technologies shows you transfection best practices and recommendations for success using reagents and the Neon(tm) Transfection System. You'll get tips on ...
    12-6-2012
  • Optimizing Cell Lines Improves Production-Really!
    ... Bi-Hex integrates novel vectors, serum-free transfection, optimized host cells, serum-free ... occurs in HEK cells, which are more amenable than CHO to lipid-based transfection methods. ...
    11-15-2012
  • Automated HT Reverse Transfection of Plasmid DNA
    Results from Studies with Two New Reagents Showed Compatibility with Accepted Methods Figure 1. Formation of the transfection complex, cell seeding, and transfection were performed ...
    11-15-2012
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    Journal Articles

  • MicroRNA-1 and MicroRNA-206 Improve Differentiation Potential...
    Merel Koning, Paul M.N. Werker, Daisy W.J. van der Schaft, Ruud A. Bank, Martin C. Harmsen
    Tissue Engineering, Part A
    As a proof of concept, this was also investigated in a three-dimensional bioartificial muscle construct. After transfection with microRNA-1, the number of Pax7 expressing cells ...
  • Effects of Glucocorticoid Receptor Small Interfering RNA Delivered...
    Liu Hong, Na Wei, Vijaya Joshi, Yang Yu, NaJung Kim, Yogita Krishnamachari, Qiang Zhang, Aliasger K. Salem
    Tissue Engineering, Part A
    Transfection of GR siRNA significantly downregulates GR and upregulates the expression of ... Under adipogenic differentiation, MSC proliferated in vitro with siRNA transfection, ...
  • Extending the Activities of Human Nucleus Pulposus Cells with...
    JianHong Wu, DeLi Wang, Chao Zhang, Chaofeng Wang, Yan Zhang, Hongkui Xin, Qing He, Dike Ruan
    Tissue Engineering, Part A
    evidence of polyploidy or oncogenicity in nude mouse. The results showed that this transfection model might provide a resource cells necessary for the biological treatment of ...
  • Vascular Endothelial Growth Factor Inhibits Bone Morphogenetic...
    Björn H. Schönmeyr, Marc Soares, Tomer Avraham, Nicholas W. Clavin, Fredrik Gewalli, Babak J. Mehrara
    Tissue Engineering, Part A
    The purpose of these experiments was therefore to evaluate the effect of dual transfection of these growth factors and potential mechanisms of interaction on gene expression and ...

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