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  • 6 Considerations for Multiplex qPCR Setup
    of amplification are critical. Multiplex PCR can be used to measure the expression ... a different set of primers, and a uniquely labeled probe distinguishes each PCR amplicon. ...
    5-13-2013
  • Companion Diagnostics: 52 Pick-Up
    ... Real-time PCR test intended to assist in selecting melanoma patients whose tumors carry ... is being considered. therascreen KRAS RGQ PCR Kit Sponsor/Developer: Qiagen Intended Use/...
    5-9-2013
  • Cancer Diagnostics, Pushing the Boundaries
    ... Using a Sanger sequencing assay on genomic DNA extracted from FFPE tissue sections and amplified with PCR they could detect known hot-spot mutations in specific exons. The ...
    5-8-2013
  • Biomarkers Take Center Stage
    ... type will have two demethylated copies of a certain gene locus, allowing them to be enumerated by quantitative PCR. The biggest challenge is finding that unique epigenetic marker. ...
    5-1-2013
  • Gene Expression Analysis in Living Cells
    ... Correlates with qPCR Results Figure 3. Probe detection of mRNA levels correlates to RT-PCR ... as quantitated using SmartFlare; both sets of results correlate to their RT-PCR levels. ...
    5-1-2013
  • The Globalization of IVD Companies
    The third was investment in enabling technologies like next-generation sequencing and digital PCR. Out of the list, entering emerging markets is broadly applicable across all IVD ...
    5-1-2013
  • An Array of Genetic Tools from Gene Link
    Under the "tools" heading one will find an oligo Tm calculator, tools to calculate concentration for oligo resuspension and dilution, typical reaction and cycler conditions for PCR...
    5-1-2013
  • Testing for Residual Host Cell Protein & DNA
    ... Kibbey: Technologies used to detect residual HCP differ from those used to test for residual DNA. While several methods can be applied for host-cell DNA detection, PCR is one of ...
    5-1-2013
  • Detecting Acquired Drug Resistance in Cancer
    ... The blood analysis was performed using Inostics' BEAMing digital PCR technology. Originally developed in the laboratory of Bert Vogelstein, M.D., at Johns Hopkins, BEAMing ...
    4-29-2013
  • $20M in Financing Helps RainDance Summon ThunderStorms for Myriad
    system for targeted next-generation sequencing, and the recently launched RainDrop(tm) digital PCR system for multiplexed quantitative detection of circulating nucleic acids. ...
    4-29-2013
  • Responding to H7N9 Threat, FDA Issues Emergency Use Authorization...
    Human Influenza Virus Real-Time RT-PCR Diagnostic Panel-Influenza A/H7 Assay in ... the CDC Human Influenza Virus Real-Time RT-PCR Diagnostic Panel-Influenza A/H7 (Eurasian ...
    4-26-2013
  • 5 Tips to Help You Get PCR Automation Right
    Find out how to make the right decisions for your lab. The Hamilton PCR Setup STARlet® workstation is an example of a device researchers can use for automated PCR sample prep. PCR ...
    4-19-2013
  • Literature Review: Bacteria Reprogram Host Cells in Order to...
    purified by FACS with anti-p75 antibody (B). (C) RT-PCR of purified p75 + Schwann cells. ... Scale bars: (D) 50 μm, (F, G) 10 μm. RT-PCR, reverse transcription polymerase chain ...
    4-18-2013
  • Illumina Partnership Eyes Expanded Presence in China
    Featuring single-tube sample preparation and genome-wide amplification without PCR or ... analyzing gene expression using real-time PCR. Key to the NuPCR reaction is the NuZyme ...
    4-18-2013
  • Advances in RNAi Tools and Technologies
    ... Integrated shRNA sequences are PCR amplified and amplicons are analyzed by next-generation ... at the transduction step, the PCR amplification step, and data analysis by ...
    4-15-2013
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    Journal Articles

  • Natural Infection of Nyssomyia neivai by Leishmania (Viannia...
    Diego Molina Oliveira, Karin Rosi Reinhold-Castro, Marcos Vinícius Zandonadi Bernal, Cristiane Machado de Oliveira Legriffon, Maria Valdrinez Campana Lonardoni, Ueslei Teodoro, Thaís Gomes Verzignassi Silveira
    Vector-Borne and Zoonotic Diseases
    cutaneous leishmaniasis was analyzed using multiplex polymerase chain reaction (PCR). ... Multiplex PCR analyses using MP3H-MP1L and 5Llcac-3Llcac primers showed the presence of ...
  • A Haplotype at the UCP1 Gene Locus Contributes to Genetic Risk...
    Karani S. Vimaleswaran, Venkatesan Radha, Saurabh Ghosh, Partha P. Majumder, M.R.S. Rao, Viswanathan Mohan
    Metabolic Syndrome and Related Disorders
    The polymorphisms were genotyped using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Linkage disequilibrium (LD) was estimated from the ...
  • First Molecular Detection of Anaplasma phagocytophilum in European...
    Bronislava Víchová, Viktória Majláthová, Mária Nováková, Martin Straka, Branislav Pet'ko
    Vector-Borne and Zoonotic Diseases
    Tissue samples from European brown bears (Ursus arctos) were tested for the presence of A. phagocytophilum DNA by a PCR amplification of 16S rRNA gene. The results of our study ...
  • Prevalence of Five Pathogenic Agents in Questing Ixodes ricinus...
    Violaine Cotté, Sarah Bonnet, Martine Cote, Muriel Vayssier-Taussat
    Vector-Borne and Zoonotic Diseases
    (PCR) amplification were performed on individual nymphal, male, and female I. ricinus ticks. Amplification from 1 tick among the 572 samples (0.2%) resulted in PCR products ...

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