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  • Twin Study Elucidates Epigenetics' Role in Autism
    The team studied DNA methylation, which acts to block the genetic sequences that drive gene expression, silencing gene activity. They examined DNA methylation at over 27,000 sites ...
    4-23-2013
  • Epigenetics Opens New Avenues for DNA Research
    ... to cause epigenetic changes that include aberrant DNA methylation and post-translational histone modifications, causing changes in chromosomal condensation and gene silencing. ...
    4-1-2013
  • Flipping the Genetic Switch
    ... more highly methylated CpG islands (genomic areas with large numbers of cytosine-guanine pairs), which are often associated with gene transcriptional silencing of promoters. ...
    3-28-2013
  • DNA Methylation Rules: Studying Epigenetics with New Tools
    In particular, de novo methylation of tumor suppressor gene promoters occurs frequently in cancers, thereby silencing them and promoting transformation. Cytosine hydroxymethylation ...
    2-6-2013
  • Costly Cats: Dispelling Allergies-to-Animals Mythology
    ... Using a gene-silencing strategy, the investigators specifically inhibited the expression of MR on human monocyte-derived DCs. They showed that silencing MR expression on ...
    12-12-2012
  • Overcoming Drawbacks of Gene Silencing with RNAi
    Tools for Effective Validation and Utilization Gene silencing by RNA interference has become a key tool in research and drug discovery since its discovery by Andrew Fire and Craig ...
    12-1-2012
  • Understanding Genetic Risk for Diabetes
    the scientists can manipulate gene activity in the most widely used mouse ... The paper is titled "PTPN22 Silencing in the NOD Model Indicates the Type 1 ...
    11-29-2012
  • RNAi's Enabling Powers
    ... or established cell lines, adherent or in suspension, all cells tested readily take up the sd-rxRNA based on their structure and show significant gene silencing, greater than 70%." ...
    11-1-2012
  • Epigenetics Study Identifies Gastric Cancer Subtype
    Over 97% of these gene-silencing methylated CpG sites were within gene promotor regions. The studies also identified long-range regions of epigenetic silencing (LRESs) within the ...
    10-18-2012
  • Gene Expression Analysis Reshapes Biomedical Research
    ... Jones and colleagues used gene expression meta-analysis to define the regions that are dependent on DNA methylation-dependent gene silencing. Their strategy helped identify a set ...
    10-1-2012
  • The Empty (and Misleading) Promises of Do-It-Yourself Genetic...
    ... that DNA sequence is only the first stage of gene expression. One must consider such events as RNA splicing, RNA silencing, and protein modifications (to name a few) if one ...
    9-6-2012
  • Honing In On Gene Delivery Targets
    ... things, that they need not worry about the random integration of vector (and all that entails in terms of transgene or endogenous gene silencing or endogenous gene activation). ...
    9-1-2012
  • RNAi Technology
    In 1990, a plant geneticist, Dr. Richard Jorgensen, serendipitously discovered post-transcriptional gene silencing while working with petunias. Eight years later Drs. Andrew Fire ...
    8-14-2012
  • Exposing the Roots of Chemotherapy Resistance
    or those that expressed a WNT16B-silencing shRNA. Tumors growing in the presence of ... exposed to genotoxic stress was induced through NF-κB binding to the WNT16B gene. ...
    8-6-2012
  • 9 Tips for Optimal siRNA Transfection
    The better the siRNA, the lower the concentration needed for high silencing. Check the half-lives of the protein and mRNA of interest and measure gene silencing accordingly 24 to ...
    7-27-2012
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    Journal Articles

  • Chromatin Insulator Elements Block Transgene Silencing in Engineered...
    Chad C. MacArthur, Haipeng Xue, Dennis Van Hoof, Pauline T. Lieu, Miroslav Dudas, Andrew Fontes, Andrzej Swistowski, Thomas Touboul, Rina Seerke, Louise C. Laurent, Jeanne F. Loring, Michael S. German, Xianmin Zeng, Mahendra S. Rao, Uma Lakshmipathy, Jonathan D. Chesnut, Ying Liu
    Stem Cells and Development
    lineages, reduction in reporter expression was observed, indicating transgene silencing. ... The green fluorescent protein (GFP) gene was driven by EF1a or CMV early enhancer/chicken ...
  • Generation of High-Level Stable Transgene Expressing Human Embryonic...
    Ken Kwok-Keung Chan, Selena Meiyun Wu, Peter Morin Nissom, Steve K.W. Oh, Andre B.H. Choo
    Stem Cells and Development
    (CHEF1) promoter robustly drove reporter gene expression higher than the human ... in differentiated embryoid bodies (EBs), and in teratomas without transgene silencing. ...
  • βig-h3 Potentiates the Profibrogenic Effect of TGFβ Signaling...
    Carlos I Lorda-Diez, Juan A Montero, Manuel J Díaz-Mendoza, Juan A García-Porrero, Juan M Hurlé
    Tissue Engineering, Part A
    Gene expression analysis in several physiological and experimental paradigms suggests that ... of BIG-H3 is lost following Hif-1a silencing with shRNA. Collectively, our findings ...
  • Stable Plasmid-based siRNA Silencing of Gene Expression in Human...
    Yi-Ping Liu, Svetlana V. Dambaeva, Oksana V. Dovzhenko, Mark A. Garthwaite, Thaddeus G. Golos
    Stem Cells and Development
    Stable Plasmid-based siRNA Silencing of Gene Expression in Human Embryonic Stem Cells Stem Cells and Development RNA interference (RNAi) using short inhibitory RNAs (siRNAs) has ...

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