This new wound-healing array for automated label-free measurement of cell migration uses electric cell-substrate impedance sensing (ECIS) instruments. As in the ECIS migration assay, weak AC current is used to noninvasively monitor cell migration after an elevated electric field is used to wound cells on small gold electrodes. The new arrays feature line electrodes that measure 150 by 667 micrometers. These arrays produce wounds that mimic the shape of traditional mechanical scrapes and return migration data in a shorter time than the standard ECIS circular electrodes. Measurements are automated and occur without opening the incubator door. The new arrays are available in eight-well format and will soon be available with 96 wells.