The Clarity™ Western ECL substrate is a chemiluminescent detection reagent. Suited to meet the needs of researchers utilizing Western blotting, Clarity substrate provides good results for both high- and low-expression proteins using either film or digital detection systems. The Clarity Western ECL substrate was designed for blots using nitrocellulose, PVFD, and low-fluorescence PVDF and can be used to detect both high- and low-abundance proteins when using film or CCD imaging systems for blot detection. Clarity ECL substrate has a one-year shelf life and is stored at room temperature.