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June 1, 2013 (Vol. 33 , No. 11)

Sequencing System

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Pacific Biosciences

The PacBio RS II allows scientists to generate finished genome assemblies, reveal and understand epigenomes, and characterize genomic variation. Based on the SMRT sequencing technology, the RS II can generate average read lengths of 5,000 base pairs, with the longest reads above 20,000 base pairs in length. The PacBio RS II system, including consumables and software, provides an end-to-end workflow for SMRT sequencing in research areas including infectious disease and microbiology, agriculture, and complex genetic diseases with repeat expansions. With the PacBio RS II, the number of simultaneously observable sequencing reactions is doubled, thereby doubling the system’s throughput.

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