Lasergene 10 is a Sanger and next-generation sequence analysis and assembly software upgrade with new features to its desktop software suite for molecular biologists. Among other changes, Lasergene 10 introduces SeqNinja, a tool for manipulating and modifying large or complex sets of genomic data or editing sequence data and annotations across large datasets. Other new features supported in Lasergene 10 include extended next-gen sequence assembly and analysis support for Ion Torrent and Pacific Biosciences data and applications; incorporation of dbSNP, Cosmic, and GERP database information into sequence assembly and analysis projects; the ability to create custom SNP tables; and detection of splice site sequence variations.