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November 15, 2011 (Vol. 31 , No. 20)


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The Ready One-Step RT-PCR kit includes Ready One-Step reverse transcriptase, which consists of M-MLV and a buffer that delivers cDNA synthesis from rare and abundant transcripts. The kit also contains Ready One-Step RT-PCR Mix, 2X comprised of reaction buffer, Extender™ DNA Polymerase Blend, dNTPs, an electrophoresis tracking dye, and a nonmutagenic EZ-Vision® visualization dye. The combined reverse transcription and PCR reaction is prepared in a single tube using as little as 1 pg of RNA template and user-supplied primers. Upon completion of amplification, samples can be loaded directly onto an agarose gel. The EZ-Vision dye works with standard UV illumination to reveal amplification products after electrophoresis without additional post-staining and destaining steps.

  • Amresco
  • 6681 Cochran
  • Solon OH, 44139-0098

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