The PowerMicrobiome™ RNA isolation kit is designed for purification of total RNA from samples high in PCR inhibitors including stool, gut material, and biosolids. Inhibitor Removal Technology® (IRT) ensures complete removal of inhibitory substances from digested food, heme from lysed red blood cells abundant in stool, and other PCR inhibitors. The RNA isolated is of good quality and ready to use in downstream applications, including RT-qPCR and sequencing. Optional removal of co-isolated DNA can be performed using an on-column procedure with reagents provided in the kit.