The GoTaq® Probe PCR Master Mix, GoTaq Probe 1-Step RT-qPCR system, and GoTaq Probe 2-Step RT-qPCR system are ready-to-use, 2x master mixes that can simplify the preparation of reactions for qPCR using hydrolysis probe detection. They are designed for sensitive sequence detection and quantification over a range of DNA or RNA targets, gene expression analysis, detection of sequence variants, and fluorescent detection. GoTaq Probe systems are compatible with any real-time PCR instrument using standard- or fast-cycling programs. The 1-step and 2-step systems also include GoScript™ Reverse Transcriptase to enable efficient synthesis of first-strand cDNA in preparation for PCR amplification.