Designed for use as a critical component in ELISA-based test procedures, the CENP-B antigen can be utilized to detect anticentromere autoantibodies in human serum. A recombinant, full-length human-sequence protein expressed and purified in Sf9 insect cells constitutes this CENP-B antigen. The antigen demonstrates a product purity level in excess of 90%, as assessed by SDS gel electrophoresis, while also demonstrating specificity and activity. With reliable lot-to-lot consistency and long shelf life stability, the antigen is offered in operator-oriented fill formats of 0.2 mg and 1.0 mg vials, with customer-specific and bulk packaging configurations also available. This product would be beneficial to clinical researchers, medical laboratory professionals, and manufacturers of in vitro diagnostic immunoassay kits for autoimmune conditions.