The CFX qualification plate verifies the performance of any CFX real-time PCR system. Preloaded with a master mix containing SsoAdvanced™ SYBR® Green supermix, the plate provides a five-point standard curve, two unknown groups, and no-template controls, allowing for the determination of a pass/fail instrument performance result. Included in the CFX Manager™ software is the qualification plate check tool for analyzing the data. The tool uses an algorithm to determine a pass/fail performance result based on two criteria: twofold discrimination with a high confidence level and single-product melt peaks for the two unknowns.