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April 15, 2012 (Vol. 32 , No. 8)

qPCR Primers

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Integrated DNA Technologies (IDT)

The PrimeTime® qPCR Primers are the same primer pairs used in PrimeTime qPCR Assays, selected to detect and quantify gene expression in human, mouse, and rat transcriptomes. The shipped product consists of a pair of premixed primers, normalized to 5 nmoles, supplying researchers with enough primer for 500 20-µL qPCR reactions with an efficiency of more than 90%. Each set of primers incorporates sequence information, along with melting temperature (Tm) and secondary structure predictions. Each is BLAST searched to eliminate off-target effects and to avoid the impact of single nucleotide polymorphisms.

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