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September 15, 2011 (Vol. 31 , No. 16)

qPCR Data-Analysis Software

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New qPCR data-analysis software is available to promote fast processing of high-throughput qPCR data. The new software introduces a new data import wizard that enables automatic annotation of different miRNA assays and direct import of instrument data files. The software is equally suitable for low- and high-throughput data analysis as it allows for batch-mode analysis of several hundred data files. The software incorporates quality control assays ensuring interplate calibration and quality checks for potential presence of sample derived inhibitors of the RT and PCR reactions. Normalization is made to stably expressed reference genes or, if preferred, to the global mean of all expressed microRNAs. Multiple statistical analyses, from simple t-tests to advanced PCA and heat map plots, can be applied and can automatically create publication-ready figures. It is also possible to retrieve database information from various databases such as miRBase, miRtarget, and miRtissue.

  • Exiqon
  • Skelstedet 16
  • Vedbaek , DK-2950

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