PrimeTime predesigned qPCR assays are designed to work for human, mouse, and rat transcriptomes. Users can search and select from the online database of thousands of assays. All assays are designed to span exons, protect against nonspecific amplifications, avoid known SNPs, and minimize the impact of secondary structure. Consisting of two primers and a hydrolysis probe, all three components are shipped in a single tube. Users can select from five different dye/quencher combinations and choose a premixed primer to probe ratio from 1:1 to 4:1, thereby maximizing multiplexing capabilities. The design process uses the most current, clean, and complete target sequence information available to generate accurate Tm predictions and protect against off-target amplifications.