Super G Blocking Buffer, a reagent for use with protein microarrays, is designed to prevent non-specific protein binding to nitrocellulose film slides. The reagent is useful for promoting low fluorescence background. Results with Super G show reduction in fluorescence background while maintaining the high protein-binding capacity of porous nitrocellulose. Super G is available as a 1x-ready-to-use solution in 100 mL or 500 mL quantities.