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March 1, 2012 (Vol. 32 , No. 5)

Protein Fractionation Kit

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Thermo Fisher Scientific

The Thermo Scientific Subcellular Protein Fractionation Kit for Tissue enables the step-wise preparation of cytoplasmic, membrane, soluble nuclear, chromatin-bound, and cytoskeletal protein fractions with less than 15% cross-contamination in two hours. The resultant samples are useful for downstream analyses, including Western blotting, protein quantitation, electrophoretic mobility shift, and reporter gene assays. The kit is suitable for use with a variety of tissue types including liver, heart, brain, kidney, lung, and spleen.

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