StrobeLock is a time-correlated single-photon counting measurement option. The imaging modes include fluorescence lifetime imaging and time-resolved luminescence microscopy, which can be integrated with the alpha300 and alpha500 microscope series. StrobeLock can facilitate the acquisition of additional material contrasts hidden in the time function of a fluorescence or luminescence signal and allows them to be linked with Raman, SNOM, or AFM imaging. StrobeLock is composed of a pulsed excitation laser system combined with a time-correlated single-photon counting detector. Microscope users can switch between time-resolved and conventional mode.