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August 1, 2012 (Vol. 32 , No. 14)

PCR Supermixes

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Bio-Rad Laboratories

Two iTaq Universal supermixes are now available that are compatible with any real-time PCR instrument. These products enable users to obtain high-quality data across all real-time quantitative PCR (qPCR) applications. The iTaq Universal SYBR® Green supermix and the iTaq Universal Probes supermix are both 2x concentrated, ready-to-use reaction master mixes that contain a blend of multiple passive reference dyes. They are useful for researchers using real-time PCR instruments that require ROX fluorescent dyes. The SYBR Green supermix is optimized for dye-based qPCR and contains SYBR Green I dye, whereas the Probes supermix is formulated for probe-based qPCR (simplex or duplex).

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