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January 15, 2014 (Vol. 34 , No. 2)

PCR-Free DNA Library Kit

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Swift Biosciences

DNA library preparation via Accel-NGS, previously an option for Ion Torrent platforms, is now available for Illumina next-generation sequencing (NGS) systems. Accel-NGS can produce high complexity, PCR-free libraries with as little as 250 ng of input DNA. That is, despite low DNA input levels, the product can provide high sequence quality and coverage with very low chimera formation. The product’s protocol consists of five steps and can be executed quickly, requiring about 90 minutes from start to finish. In addition, the Accel-NGS PCR-free DNA Library Kit does not require intact double-stranded DNA, making it compatible with damaged samples. Optional single- and dual-indexing sets are available.

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