The Attune NxT Acoustic Focusing Cytometer uses an acoustic focusing technology to increase detection sensitivity and throughput. Acoustic focusing enables higher control of the cells as they pass through the interrogation point, resulting in faster and more precise sample interrogation capabilities. The Attune NxT uses sound waves, as opposed to hydrodynamics, to focus cells past the laser detection system for analysis. Multiple fluorescent proteins can be detected with an optional 561 nm laser. It can also be easily configured to run with one to four lasers, enabling it to detect up to 14 colors in a sample and to scale with changing research needs and budgetary demands.