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January 15, 2014 (Vol. 34 , No. 2)

Mutation Analysis Assays for Digital PCR

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Bio-Rad Laboratories

Newly released PrimePCR assays for Droplet Digital PCR (ddPCR) systems expand the PrimePCR collection by offering by an additional 46 mutation detection assays and 323 copy number assays. ddPCR technology can enable the detection of one mutant molecule in the background of 100,000 wild-type sequences (0.001%). In addition, not only can ddPCR assays enable the quantitative discrimination required to resolve small-fold changes in gene copy numbers, they can also detect subsequent changes in the expression of target genes. PrimePCR ddPCR assays are available in multiple reaction sizes and are compatible with both the QX100 and QX200 platforms using the ddPCR supermix for probes.

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