Biacore™ T200 Software v.2.0 enhances the capabilities of Biacore T200, offering researchers a tool for processing molecular interaction data. Together, they can facilitate label-free screening of low-molecular-weight compounds and low-abundance proteins, giving detailed binding profiles on molecule-drug candidate mechanisms of action that ultimately assist in earlier lead selection and optimization. The new software can simplify and speed up the generation and transformation of raw label-free interaction data into high-quality information, reducing the time needed for screening and hit validation. It also encompasses algorithms for ranking and qualification of binders versus nonbinders, and enables comparison of datasets through normalization of sample responses by a number of means.