Version 2.5 of the MetaMorph® NX microscopy automation and image analysis software now includes modules for the study of neuronal morphology and fiber structures, support for targeted illumination devices, and a high-speed image acquisition mode. The Neurite Tracing Application Module can simplify the tasks associated with neuron research, while the Fiber Tracing Application Module can help make sense of interwoven fiber structures. Added support for targeted illumination devices enables synchronization with regions of interest or an exact position in the image, and the new high-speed image acquisition mode allows researchers to record large numbers of rapid changes in cellular function and behavior.