The XFe96 Spheroid Microplate is a 96-well plate featuring geometry that enables functional, metabolic measurements of individual spheroids for the study of three-dimensional cell culture, which more closely mimics in vivo conditions. Relevant for tumor studies, the real-time metabolism of multicellular spheroids can be analyzed and used for evaluating the metabolic response to therapeutic agents. Using the XFe96 Spheroid Microplate and the XFe96 Extracellular Flux Analyzer, scientists can obtain sensitive and reproducible data from individual spheroids with high specificity. The XFe96 Extracellular Flux Analyzer simultaneously measures the two major energy-producing pathways within the cell—mitochondirial respiration and glycolysis—in a microplate, in real time. The XFe96 Spheroid FluxPak contains six cartridges, six microplates, and calibrant.