An analysis module for the ambr™ microbioreactor system is now available, providing automated pH calibration for enhanced bioprocess control. The module can automate at-line pH measurement and calibration to produce scalable results by enabling feedback and pH control of cultures. It provides automated at-line pH calibration during culture set-up and pH recalibration at any time during the run. The analysis module is designed to use small culture samples, reducing the impact on the culture volume and enabling hands-free daily pH checks. The unit comes with a reagent pack including pH reference buffers for fully automated self-calibration and a low volume-pH.