GoTaq® Long PCR Master Mix is an enzyme mixture for improved long-range PCR. The new kit enhances PCR yield, sensitivity, and specificity and enables amplification of up to 40 kb from lambda DNA or 30 kb from human genomic DNA. The GoTaq Long PCR Master Mix components have been engineered with hot start formulation. The new master mix is useful for cloning genes, mutational analysis, and DNA sequencing. GoTaq Long PCR Master Mix contains the polymerase and proofreading enzyme mixture, dNTPs, and MgCl2. Also provided are control primers, human genomic control DNA, and nuclease-free water.