SimLipid facilitates analysis of lipid mass spectrometric data and offers user interfaces to perform complex lipid MS data analysis. The program profiles lipids by matching the experimental mass spectrometric data with its own database, composed of nine lipid classes and 1,948 lipid species. It retrieves lipids that correspond to the observed m/z value and user-specified filter criteria. Alongside the probable lipid structure, information such as lipid ID, lipid abbreviation, systematic name, lipid category, lipid mass, chemical formula, and other database links is also made available. SimLipid annotates the mass spectra with the abbreviation of identified lipid structures, and the annotated spectra can be exported as image files.